Long�R3IGF-I is an analog of human IGF-I
� It is a superior alternative to insulin in serum-free media.
� It increases protein production by cells in culture medium.
� It increases cell viability by inhibiting apoptosis.
� It has a longer half-life in cell culture than insulin.
� It is readily available.
� There is secure and ample manufacturing capacity at GroPep Limited.
� No animal- or human- derived material is used in the manufacture or storage of
� Long�R3IGF-I is already being used in the manufacture of three (3)
biopharmaceuticals approved by FDA and EMEA.
Frequently Asked Questions
What cell types will respond to Long�R3IGF-I?
All cells that have a Type I IGF receptor will potentially respond. Most
commercially used cells including CHO, fibroblasts and hybridomas have a type I
IGF receptor. All cells which respond to pharmacological concentrations of
insulin (>1 mg/liter) will respond to Long�R3IGF-I (10-50 mg/liter).
Is storage of the stock solution at 4�C acceptable?
How long is the stock solution stable for under these storage conditions?
Liquid stability data shows that Long�R3IGF-I is stable for 3 years (-20�C to
37�C). Therefore, the stock solution should be stable at 4�C for 3 years.
What type of preparation is available?
Liquid formulation, preferable for GMP production.
Freeze dried preparation.
Is Long�R3IGF-I stable?
Re-test date for freeze-dried peptide is 3 years. Liquid formulation stability
studies have recently been completed. It is stable for 3 years (-20�C to +37�C).
We have data indicating stability in media at 4�C for 1 year.
Here is an article written by a self-experimenter.
December 15, 2000
Answer: What a perfect question! You actually have talked to just the right
person. I have a business associate that worked for the company that produces
this in Australia. Several years ago, I ordered 10mg of Long R3 IGF-1 and used
it for several months. What I found out was truly amazing.
Before I tell you about my results, let me tell you that if you are going to use
IGF-1 then make sure it is the Long R3 version! Let me explain. Regular IGF-1
like what is produced in your body is transported around connected to binding
proteins. There are quite a few of these and their main purpose is to grab ahold
of the IGF-1 peptide and keep it from being quickly degraded. Without these
binding proteins, all of the IGF-1 would be metabolized in the body within a few
minutes. The problem (at least it seems like a problem but might actually be a
good thing) is that these binding proteins basically prevent the IGF-1 from
performing its function. As long as IGF-1 is attached to the binding protein it
cannot do the cool stuff that it wants to do. Regular IGF-1 must be released
from its binding protein in order to accomplish its mission. Part of the problem
is that much of the IGF-1 is degraded before it is released (seems like much is
wasted doesn�t it?)
With Long R3 IGF-1 this problem doesn�t exist. Understand that the Long R3
version does not bind to the various binding proteins. It is free to move
throughout your body and immediately start doing all the cool stuff that it
wants to do. Again, understand that the Long R3 version is several orders of
magnitude stronger than regular IGF-1.
If you would happen to use regular IGF-1, you would need several milligrams per
day in order to get the desired effect. With the Long R3 version, you need only
microgram quantities. Long R3 is also inherently MUCH cheaper to produce. What I
am saying is that for the average person, regular IGF-1 is not practical-it is
too expensive and you need to use too much. With Long R3 IGF-1, the price to
results ratio is pretty good!
Something else I want to explain is how I went about preparing it for injection
into my body. Unfortunately, this is not easy and the average person will have a
hard time doing it. At the time, I worked in a sophisticated lab which had all
of the necessary equipment. I ordered 10mg of Long R3 IGF-1 and it came in a
single flip-top vial. 10mg might not seem like much but believe me, when it
comes to Long R3 IGF-1, it is a ton! Some people might say to just add saline to
the vial, keep it in the fridge and inject it when necessary. However, this will
not work well because the IGF-1 is not highly stable and will degrade in an
aqueous environment. 10mg was enough for many months and I needed a way that
would allow the IGF-1 to remain potent during this entire time. I did my
research and developed my method. I ordered what is known as microvials and
sterilized them. I then diluted the IGF-1 with sterile water and added just a
tad of acid to increase stability. Although it took quite a while, I then used a
micropipette and alliquotted an amount of solution that contained 50mcg into one
of my microvials. I closed the microvial and then froze it in a deep freezer.
When I was ready to inject, I took out one or more of my microvials, thawed it
out, combined it with saline and injected it.
When I first started taking Long R3 IGF-1, I used 50mcg every other day.
Amazingly, within days, I started noticing some effects in my body. I felt super
hungry all of the time and just felt �anabolic�. I can�t describe this feeling
except to say that it was very similar to being on anabolic steroids (I wasn�t
on at the time). Within one month, I gained almost 17 pounds of fairly lean
mass! After the first month, something happened though and I noticed that it
didn�t seem to be working that well. I upped the dosage several times over the
next month to keep up the desired effects. On the third month, I was using
several hundred micrograms per day but wasn�t noticing any further gains. All in
all, I gained about 20 pounds of pretty solid mass!
Please notice that almost all of my gains were within the first month of taking
the Long R3 IGF-1. After this first month, my gains slowed down considerably and
eventually stopped altogether even though I was taking high dosages. Why did
From all of my research, I suppose one of two things might have happened to
prevent me from making further gains. What I truly suspect is that the Long R3
IGF-1 downregulates the amount of binding proteins being produced by my body
(research confirms this). When I first started to inject the IGF-1, I was
supplementing my own body�s IGF-1. I not only had my own IGF-1 working
throughout the day but I had the potent surges of Long R3 IGF-1 that I would
inject. Over time though, the binding proteins were downregulated. Of course my
body continued to produce some (albeit less) IGF-1, however, because there were
very little or no binding proteins it was quickly degraded. From what I can
tell, I was in a state where 95% of the day my body did not have the benefits of
IGF-1. Basically, it got what it got when I injected the Long R3 version.
The other possibility is that I built up antibodies to the Long R3 IGF-1 which
basically sought out and destroyed what I injected. Although possible, I don�t
believe this actually happened because it is not supported by research. I have
seen no evidence which suggests that Long R3 IGF-1 causes antibody production.
To fix the above problem, one would have to cycle the Long R3 IGF-1. The best
thing would probably be to take it every other month. This would allow your own
body�s IGF-1 and binding proteins to return to normal.
Overall, I had a good experience with Long R3 IGF-1. The results were different
than with steroids. I have noticed that steroids cause preferential growth of
certain muscles, especially those that are stressed (as in lifting). The IGF-1
though seemed to cause my entire body to get a little thicker. I guess IGF-1 is
less compensatory in nature and exerts a more whole-body anabolicity.
Would I recommend IGF-1? To the right person who is very careful and knows what
he�s doing and has a good background in the sciences and has access to a good
lab, YES! However, you can tell that I have listed many prerequisites to using
it. For the average Joe, I believe is is just too complicated to be safe.
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